Abstract. CARDONA, Eliana M.; RIOS, Luis A. and RESTREPO V., Gloria M.. EXTRACTION OF THE CAROTENOID LYCOPENE FROM CHONTO TOMATO. : Extracción y estabilidad del licopeno de tomate en sistemas modelo: Estabilidad del licopeno en condiciones de iluminación y oscuridad. ESA Extraccion fraccionada de carotenoides de fuentes naturales con alto contenido en licopeno mediante fluidos.

Author: JoJora Mezijar
Country: Mozambique
Language: English (Spanish)
Genre: Science
Published (Last): 26 September 2004
Pages: 455
PDF File Size: 11.58 Mb
ePub File Size: 6.9 Mb
ISBN: 997-7-93051-931-9
Downloads: 70183
Price: Free* [*Free Regsitration Required]
Uploader: Faesar

The crude carotenoids shown in figure 1A and the precipitated lycopene and supernatant shown in figure 1B were developed with toluene-hexane 1: The comparative protective effects of degraded carrageenin and aluminium hydroxide on experimentally produced peptic ulceration. The HPLC chromatograms of extracts and anti-solvent licoeno are shown in figure 3. Crystallization is therefore, an aspect of precipitation, obtained through a variation in the solubility conditions of the solute licopeni the solvent, as compared to precipitation resulting from a chemical reaction O’Grady et al.

Un método simple para aislar licopeno, a partir de Lycopersicon esculentum

Journal of National Cancer Institute Analysis by high-performance liquid chromatography for the quantification of lycopene. Sample preparation and materials. Concentrations of lycopene in L. Effects of modifiers on the profile of lycopene extracted from tomato skins by supercritical Co 2.

ES2183471T3 – Procedimiento para la extraccion de licopeno. – Google Patents

Effect of high-pressure exrraccion on the carotenoid composition and the radical scavenging activity of persimmon fruit purees. In our study, comparison of the chromatograms showed that the relative peak area of lycopene significantly increased after isolation by the anti-solvent fractionation.

It accumulates in relatively few tissues and can most easily be observed in ripe tomato, red pepper, watermelon, and red grapefruit, giving them a characteristic red pigmentation. The characterization of the lycopene extracts was performed in isocratic mode and the mobile phase used was methanol: The anti-solvent approach for crystallization is considered a powerful isolation and purification technique O’Grady et al.

The analysis of lycopene in food products may be carried out by HPLC or by direct spectrophotometry, which is the standard method for fast extraxcion of lycopene Baysal et al. Chemical Engineering Research and Design I derivati industriali del pomodoro. The previous reported lycopene recovery rates of Baysal et al. Extraction of lycopene from tomato skin with supercritical carbon dioxide: Un total de gramos de polvo liofilizado de L.

Innovative supercritical Co 2 extraction of lycopene from tomato in the presence of vegetable oil as co-solvent. In these experiments, the conventional organic solvent extracted low levels of lycopene because the hardness of the dried tomato power prevented lycopene from dissolving in the organic solvent.

Lycopene not dissolved in methanol was precipitated and recovered as a quasi-crystalline extraccio form 3. All of the lycopene recovery procedures were conducted within 2 h. To confirm the purity of the precipitated lycopene, TLC was performed Figure 2.

To our knowledge, no previous study had attempted to adapt the conventional anti-solvent precipitation technique for the isolation of lycopene. Conventional methods for the extraction of carotenoids from many various sources have used pure solvents such as dichloromethane or a mixture of polar and non-polar solvents e. Extraccion fraccionada de carotenoides de fuentes naturales con alto contenido en licopeno mediante fluidos supercriticos.

We selected the extraction solvent showing the highest extraction efficiency for the next isolation procedure. These results implied that anti-solvent precipitation using organic solvent as an anti-solvent is a far more efficient extraction method than chromatographic purification.

Lycopersicon esculentum was provided by Chal-Tomato Co. Many different techniques, such as conventional solvent extraction Sadler et al. Chromatographic purification of natural lycopene. Fractionated extraction of natural source carotenoids with a high lycopene content, using supercritical fluids. A simple method for the isolation of lycopene from Lycopersicon esculentum.


Supercritical fluid extraction with the CO 2 method has recently been proposed Vasapollo et al. A detailed and comprehensive study of amaranth Amaranthus cruentus L.

However, these isolation techniques require sensitive and complex manipulations and are time consuming. Although it has no provitamin A activity, lycopene functions as an antioxidant and quenches singlet oxygen in vitro. In conclusion, the conventional solvent extraction and anti-solvent processes are feasible methods to rapidly 2 extracccion purify lycopene extraccuon L.

The post harvest losses due to watermelons bruises or strains. Assessment of carotenoids, capsaicinoids and ascorbic acid composition of some selected pepper cultivars Capsicum annuum L. As seen figure 1methanol as an anti-solvent is added to the crude lycopene solution.

Extracción del carotenoide licopeno del tomate chonto (Lycopersicum esculentum)

In this method, a secondary solvent known as an anti-solvent or precipitant is added to the solution resulting in the reduction of the solubility of the solute in the original solvent and consequently, the generation of a super-saturation driving force Nowee et al.

The dried extract was dissolved in 20 ml of methanol: Existing methods for extracting lycopene to choose one according to the possibilities available in the environment, taking into exraccion a process without levels of chemical contaminants, mainly organic solvents that affect people, and then to determine their performance and how to purify if necessary. Postharvest Biology and Technology The effect of mixing on the metastable zone width and nucleation kinetics in the anti-solvent crystallization of benzoic acid.